Difference between revisions of "Part:BBa K3733033:Experience"

Latest revision as of 17:46, 17 October 2021

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Applications of BBa_K3733033

We transform E.coli DH5α strain with BBa_K3733033 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD₆₀₀ in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).

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Figure 1.Comparison of OD₆₀₀ corrected RFU values under induction of different concentrations of thiosulfate

User Reviews

UNIQ654756c7ddab20a1-partinfo-00000001-QINU UNIQ654756c7ddab20a1-partinfo-00000002-QINU

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 ===Applications of BBa_K3733033===
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-We transform <i>E.coli</i> DH5α strain with BBa_K3733033 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the reporter. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was spotted into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of thiosulfate were added Sodium sulfate solution. Add 2μL of sodium thiosulfate to each well for induction and measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
+We transform <i>E.coli</i> DH5α strain with BBa_K3733033 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>).
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