Difference between revisions of "Part:BBa K1969004"
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This is the full length of yeast GAL1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. In the range of 1-118 bp of the promoter, there is an upstream activating sequence mediating GAL4-dependent induction. All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p. Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression. | This is the full length of yeast GAL1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. In the range of 1-118 bp of the promoter, there is an upstream activating sequence mediating GAL4-dependent induction. All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p. Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression. | ||
| + | <html> | ||
| + | <h3>Characterization</h3> | ||
| + | <p><a href="https://2021.igem.org/Team:Toulouse_INSA-UPS">Toulouse_INSA_UPS_2021</a>contributed to the characterization of this part. The GAL1 promoter, inducible by the addition of galactose in the medium, had not been characterized before, but the team showed this year that it is functional for expression in <i>S. cerevisiae</i>. To do this the promoter was placed upon the <i>LcyE-ofCCD1</i> gene, allowing the production of α-ionone and this molecule was indentified thanks to GC-MS approaches. Check the part of the full construction <a href="https://parts.igem.org/Part:BBa_K3930003" class="pr-0" target="_blank">(BBa_K3930003)</a> for more result details.</p> | ||
| + | <p>Author = ThomasG </p> | ||
| + | <br> | ||
| + | |||
| + | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 17:40, 17 October 2021
GAL1 promoter
This is the full length of yeast GAL1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. In the range of 1-118 bp of the promoter, there is an upstream activating sequence mediating GAL4-dependent induction. All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p. Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression.
Characterization
Toulouse_INSA_UPS_2021contributed to the characterization of this part. The GAL1 promoter, inducible by the addition of galactose in the medium, had not been characterized before, but the team showed this year that it is functional for expression in S. cerevisiae. To do this the promoter was placed upon the LcyE-ofCCD1 gene, allowing the production of α-ionone and this molecule was indentified thanks to GC-MS approaches. Check the part of the full construction (BBa_K3930003) for more result details.
Author = ThomasG
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 70
- 1000COMPATIBLE WITH RFC[1000]