Difference between revisions of "Part:BBa K3784006"

 
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<partinfo>BBa_K3784006 short</partinfo>
 
<partinfo>BBa_K3784006 short</partinfo>
  
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Catalyze the production of indolepyruvate to indolepropionic acid
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Fld consists of TAA1 (BBa_K3784004), fldH (BBa_K3784003), fldB (BBa_K3784001), fldC(BBa_K3784002) and acdA(BBa_K3784000), aiming to build a pathway in the engineered bacteria to metabolize tryptophan to produce indolepropionic acid, which can consume the remaining tryptophan and strengthen the intestinal mucosal barrier.
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===Characterization===
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:11, 17 October 2021


Fld

Fld consists of TAA1 (BBa_K3784004), fldH (BBa_K3784003), fldB (BBa_K3784001), fldC(BBa_K3784002) and acdA(BBa_K3784000), aiming to build a pathway in the engineered bacteria to metabolize tryptophan to produce indolepropionic acid, which can consume the remaining tryptophan and strengthen the intestinal mucosal barrier.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal XbaI site found at 4570
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal NheI site found at 1269
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
    Illegal NotI site found at 2374
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal BglII site found at 2528
    Illegal XhoI site found at 111
    Illegal XhoI site found at 1222
    Illegal XhoI site found at 6204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal XbaI site found at 4570
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal XbaI site found at 4570
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
    Illegal AgeI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]