Difference between revisions of "Part:BBa K3776007:Design"

 
 
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===Design Notes===
 
===Design Notes===
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The sequence was from iGEM Team Marburg 2019 and was silent mutated to pass IDT screening.
+
repB and repA in this part have been codon optimized for Synechococcus elongatus UTEX 2973.
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+
−
 
+
  
 
===Source===
 
===Source===
−
 
+
It comes from the pANS plasmid in Synechococcus elongatus PCC7942.(Chen et al., 2016)
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It comes from plasmid in Synechococcus elongatus PCC7942.
+
  
 
===References===
 
===References===
 +
Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Microbiology, 162(12), 2029–2041. https://doi.org/10.1099/mic.0.000377

Latest revision as of 14:31, 17 October 2021


panS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1131
    Illegal AgeI site found at 2856
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

repB and repA in this part have been codon optimized for Synechococcus elongatus UTEX 2973.

Source

It comes from the pANS plasmid in Synechococcus elongatus PCC7942.(Chen et al., 2016)

References

Chen, Y., Taton, A., Go, M., London, R. E., Pieper, L. M., Golden, S. S., & Golden, J. W. (2016). Self-replicating shuttle vectors based on pANS, a small endogenous plasmid of the unicellular cyanobacterium Synechococcus elongatus PCC 7942. Microbiology, 162(12), 2029–2041. https://doi.org/10.1099/mic.0.000377