Difference between revisions of "Part:BBa K3861011"

 
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Tetracycline inducer system based on the tetracycline resistance mechanism found in gram-negative bacteria (Orth et al., 2000). Is is one of the most used transcriptional regulatory systems (Baron and Bujard, 2000). We use the Tet-On system where the transcription is induced in the presence of a tetracycline or tetracycline derivates (Gossen et al., 1995). The rtTA (reverse tetracycline transactivator) protein, a fusion of the TetR (tetracycline repressor) and VP16 ativation domain, can bind the TRE (tetracycline response element) only in the presence of tetracycline, thereby activating gene expression (Gossen et al., 1995).   
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Revision as of 14:22, 17 October 2021


tetR-Ptet

Tetracycline inducer system based on the tetracycline resistance mechanism found in gram-negative bacteria (Orth et al., 2000). Is is one of the most used transcriptional regulatory systems (Baron and Bujard, 2000). We use the Tet-On system where the transcription is induced in the presence of a tetracycline or tetracycline derivates (Gossen et al., 1995). The rtTA (reverse tetracycline transactivator) protein, a fusion of the TetR (tetracycline repressor) and VP16 ativation domain, can bind the TRE (tetracycline response element) only in the presence of tetracycline, thereby activating gene expression (Gossen et al., 1995).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]