Difference between revisions of "Part:BBa K3776006:Design"
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===Design Notes=== | ===Design Notes=== | ||
+ | The genes have been codon optimized for Synechococcus elongatus UTEX 2973. | ||
+ | ===Source=== | ||
+ | Pcpc560, PsbA2 and TrbcL are from the genome of Synechocystis sp PCC6803.(Li et al., 2018)(Gao et al., 2021) | ||
+ | DavB and DavA are from the 5-aminovalerate pathway in Pseudomonas putida KT2440.(Liu et al., 2014) | ||
+ | The feedback insensitive AK was mutated from WT AK in Xenorhabdus bovienii.(Qi et al., 2011) | ||
+ | ===References=== | ||
+ | Li, S., Sun, T., Xu, C., Chen, L., & Zhang, W. (2018). Development and optimization of genetic toolboxes for a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Metabolic Engineering, 48, 163–174. https://doi.org/10.1016/j.ymben.2018.06.002 | ||
+ | Gao, E. B., Kyere-Yeboah, K., Wu, J., & Qiu, H. (2021). Photoautotrophic production of p-Coumaric acid using genetically engineered Synechocystis sp. Pasteur Culture Collection 6803. Algal Research, 54, 102180. https://doi.org/10.1016/j.algal.2020.102180 | ||
− | + | Liu, P., Zhang, H., Lv, M., Hu, M., Li, Z., Gao, C., Xu, P., & Ma, C. (2014). Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase. Scientific Reports, 4(1). https://doi.org/10.1038/srep05657 | |
− | + | Qi, Q., Huang, J., Crowley, J., Ruschke, L., Goldman, B. S., Wen, L., & Rapp, W. D. (2011). Metabolically engineered soybean seed with enhanced threonine levels: biochemical characterization and seed-specific expression of lysine-insensitive variants of aspartate kinases from the enteric bacterium Xenorhabdus bovienii. Plant Biotechnology Journal, 9(2), 193–204. https://doi.org/10.1111/j.1467-7652.2010.00545.x | |
− | + | ||
− | + |
Latest revision as of 13:42, 17 October 2021
davB-davA-AK
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 800
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1889
Illegal BglII site found at 4844 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2049
Illegal NgoMIV site found at 3816
Illegal AgeI site found at 1456
Illegal AgeI site found at 1705
Illegal AgeI site found at 3040
Illegal AgeI site found at 5043 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The genes have been codon optimized for Synechococcus elongatus UTEX 2973.
Source
Pcpc560, PsbA2 and TrbcL are from the genome of Synechocystis sp PCC6803.(Li et al., 2018)(Gao et al., 2021) DavB and DavA are from the 5-aminovalerate pathway in Pseudomonas putida KT2440.(Liu et al., 2014) The feedback insensitive AK was mutated from WT AK in Xenorhabdus bovienii.(Qi et al., 2011)
References
Li, S., Sun, T., Xu, C., Chen, L., & Zhang, W. (2018). Development and optimization of genetic toolboxes for a fast-growing cyanobacterium Synechococcus elongatus UTEX 2973. Metabolic Engineering, 48, 163–174. https://doi.org/10.1016/j.ymben.2018.06.002
Gao, E. B., Kyere-Yeboah, K., Wu, J., & Qiu, H. (2021). Photoautotrophic production of p-Coumaric acid using genetically engineered Synechocystis sp. Pasteur Culture Collection 6803. Algal Research, 54, 102180. https://doi.org/10.1016/j.algal.2020.102180
Liu, P., Zhang, H., Lv, M., Hu, M., Li, Z., Gao, C., Xu, P., & Ma, C. (2014). Enzymatic production of 5-aminovalerate from l-lysine using l-lysine monooxygenase and 5-aminovaleramide amidohydrolase. Scientific Reports, 4(1). https://doi.org/10.1038/srep05657
Qi, Q., Huang, J., Crowley, J., Ruschke, L., Goldman, B. S., Wen, L., & Rapp, W. D. (2011). Metabolically engineered soybean seed with enhanced threonine levels: biochemical characterization and seed-specific expression of lysine-insensitive variants of aspartate kinases from the enteric bacterium Xenorhabdus bovienii. Plant Biotechnology Journal, 9(2), 193–204. https://doi.org/10.1111/j.1467-7652.2010.00545.x