Difference between revisions of "Part:BBa K3771020"
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<br><b style="font-size:1.3rem">Usage</b> | <br><b style="font-size:1.3rem">Usage</b> | ||
| − | <br>We ligased the <i>jju</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into | + | <br>We ligased the <i>jju</i> fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.<br> |
<br><b style="font-size:1.3rem">Characterization</b> | <br><b style="font-size:1.3rem">Characterization</b> | ||
<div style="width=100%; display:flex; align-items: center; justify-content: center;"> | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
Revision as of 12:07, 17 October 2021
PpspA-JJU
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU10.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria.[3]Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU10. JJU10 converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
Usage
We ligased the jju fragment and pspA promoter on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
Characterization
圖片描述
Reference
3. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features