Difference between revisions of "Part:BBa K3771020"
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<partinfo>BBa_K3771020 short</partinfo> | <partinfo>BBa_K3771020 short</partinfo> | ||
− | + | <html> | |
+ | <br> | ||
+ | <br><b style="font-size:1.3rem">Description</b> | ||
+ | <br>This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU10. | ||
+ | <br> | ||
+ | <br><b style="font-size:1.3rem">Biology</b> | ||
+ | |||
+ | <br>Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the <i>pspA</i> promoter, initiating expression of JJU10. JJU10 converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway. | ||
+ | <br> | ||
+ | <br><b style="font-size:1.3rem">Usage</b> | ||
+ | |||
+ | <br>We ligased the JJU10 fragment and <i>pspA</i> promoter on the pSU expression vector and transformed it into DH5alpha to complete construction of the plasmid.<br> | ||
+ | <br><b style="font-size:1.3rem">Characterization</b> | ||
+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/e/e0/T--NCKU_Tainan--colony_pcr_pspA_JJU10.jpg | ||
+ | " style="width:35%;"> | ||
+ | </div> | ||
+ | <p align="center">圖片描述</p> | ||
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+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 11:52, 17 October 2021
PpspA-JJU
Description
This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, JJU10.
Biology
Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria. [3] Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of JJU10. JJU10 converts L-cysteine into L-cystate in the taurine synthesis L-cystate pathway.
Usage
We ligased the JJU10 fragment and pspA promoter on the pSU expression vector and transformed it into DH5alpha to complete construction of the plasmid.
Characterization
![](https://2021.igem.org/wiki/images/e/e0/T--NCKU_Tainan--colony_pcr_pspA_JJU10.jpg
)
圖片描述
Sequence and Features