Difference between revisions of "Part:BBa P0140"
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<span class='h3bb'>Sequence and Features
 
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<partinfo>BBa_P0140 SequenceAndFeatures</partinfo>
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<h2>New information of TetR promoter leakage（加粗哦）</h2>
 
<h2>New information of TetR promoter leakage（加粗哦）</h2>
 
<h3>Background</h3>
 
<h3>Background</h3>
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<h3>Result</h3>
 
<h3>Result</h3>
 
<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. </p>
 
<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results. </p>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_P0140 parameters</partinfo>
 
<partinfo>BBa_P0140 parameters</partinfo>
 
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Revision as of 11:02, 17 October 2021

PoPS -> TetR [S0163]

Protein generator converting TIPS to the protein TetR. Used as the input section for Quad Part Inverter Part:BBa_Q01140.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

New information of TetR promoter leakage（加粗哦）

Background

In order to better apply the TetR promoter in the project, we characterized the leakage amount of the TetR promoter. We constructed a carotene plasmid with TetR promoter, and transformed it into yeasts, then cultured the yeasts without AHT inducer. By observing the color of the colony, the leakage of TetR promoter can be roughly determined.<p>

Result

<p>Our experimental results show that the TetR promoter is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the TetR promoter alone will affect the experimental results.