Difference between revisions of "Part:BBa J70301:Design"

 
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__NOTOC__
 
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<partinfo>BBa_J70301 short</partinfo>
 
<partinfo>BBa_J70301 short</partinfo>
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primers:
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 +
GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac,J70301-F
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GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg,J70301-R
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474647 - 474537 (reverse orientation) from NC_006055
  
 
===Source===
 
===Source===

Latest revision as of 20:39, 6 April 2009

Heat shock inducible promoter for Me. florum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Low gc content, difficult PCR primers


primers:


GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac,J70301-F

GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg,J70301-R


474647 - 474537 (reverse orientation) from NC_006055

Source

PCR from lon protease promoter of NC_006055.

References