Difference between revisions of "Part:BBa K3771013"
Line 23: Line 23:
 
<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
 
<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
 
</div>
 
</div>
−
<p align="center">Fig. 2.</p>
+
<p align="center">Fig. 2</p>
  
  

Revision as of 09:40, 17 October 2021


PlacI-OFP


Description

PlacI-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.

Biology

Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.

Characterization

Double digestion results are shown in Fig. 2.

Fig. 2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]