Difference between revisions of "Part:BBa K4090000"

Revision as of 09:36, 17 October 2021

Ice Nucleation Protein(INP)

ice nucleation protein(INP) is a kind of transmembrane protein, which can help proteins to play their role outside the membrane.

Data

File:T--T--SDSZ China--result 1-2.png According to Fig.1-1, all the fragments designed have been successfully made, including S1&S2, which are Silicatein combined with INP, and P1&P2, which are S010_pET28b-csgA-linker-mfp5-7His(pET).

File:T--SDSZ China--result 1-4.png

Fig.1-2

Fig.1-2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome.

File:T--SDSZ China--result 1-6.png

Fig.1-3

Fig.1-3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since: •Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids). •Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs).

File:T--SDSZ China--result 1-7.png

Fig.1-4

Fig.1-4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells.

File:T--SDSZ China--result 1-3-2.png

Fig.1-5

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 321
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 396
Illegal AgeI site found at 498
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 229

References

[1]ZHU T, PAULO C, MERROUN, M L, et al. Potential application of biomineralization by Synechococcus PCC8806 for concrete restoration[J]. Sedimentology, 2013,61(1):1-21 [2]Lechner, Carolin C., and Christian FW Becker. "A sequence‐function analysis of the silica precipitating silaffin R5 peptide." Journal of Peptide Science 20.2 (2014): 152-158. [3]Poulsen, Nicole, and Nils Kroger. "Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana." Journal of Biological Chemistry 279.41 (2004): 42993-42999.

@@ Line 6: / Line 6: @@
 <!-- Add more about the biology of this part here-->
 ==Data==
-[[File:T--SDSZ_China--result_1-2.png|700px|thumb|center|Fig.1-1]]
+[[File:T--T--SDSZ_China--result_1-2.png]]
 According to Fig.1-1, all the fragments designed have been successfully made, including S1&S2, which are Silicatein combined with INP, and P1&P2, which are S010_pET28b-csgA-linker-mfp5-7His(pET).
 [[File:T--SDSZ_China--result_1-4.png|700px|thumb|center|Fig.1-2]]