Difference between revisions of "Part:BBa K3771013"

 
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<br><b style="font-size:1.3rem">Description</b>
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<br><i>P<sub>lacI</sub></i>-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.<br>
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<br><b style="font-size:1.3rem">Biology</b>
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<br>Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.<br>
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<br><b style="font-size:1.3rem">Characterization</b>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/0/01/T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">
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<p align="center">Double digestion results are shown in Fig. 2.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:33, 17 October 2021


PlacI-OFP


Description


PlacI-OFP is part of the IFN-γ sensing system and is the initial composite part used to construct plasmids used in the IFN-γ sensing system experiments.


Biology


Without the presence of the LacI repressor, the LacI promoter facilitates the constitutive expression of OFP.


Characterization

<img src="T--NCKU_Tainan--composite_pLacI_OFP.jpg" style="width:35%;">

Double digestion results are shown in Fig. 2.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]