Difference between revisions of "Part:BBa K3815007"

 
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<partinfo>BBa_K3815036 short</partinfo>
 
<partinfo>BBa_K3815036 short</partinfo>
  
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The purified His-tagged peptide was recovered by Ni chromatography, and DTT was added to cleave the N-terminus of the intein.The purpose peptide is Defensine1. This is an antimicrobial peptide that that can inhibit the gram-positive bacteria. In our experiment, we used it to inhibit the bacteria in the vase water.
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This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:03, 17 October 2021


No part name specified with partinfo tag.

This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features No part name specified with partinfo tag.