Difference between revisions of "Part:BBa K3815005"

Revision as of 09:00, 17 October 2021

HIV-1 reverse transcriptase p66

This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein. In this part, the purpose protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N-end-rule.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 320
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 320
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 320
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 320
Illegal AgeI site found at 966
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3815034 short</partinfo>
-We used ELK16 system to purify the peptides. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the purpose protein. In this part, the purpose protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we  used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N end rule.
+This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein. In this part, the purpose protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N-end-rule.
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