Difference between revisions of "Part:BBa K3815002"

Revision as of 08:51, 17 October 2021

AANDENYAPHA. mutant SsrA degradation tag This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein. In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3815031 short</partinfo>
+This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein. In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
-We used ELK16 system to purify the peptides. When this fused protein are produced, ELK16 self-assembles and precipitates. After that, taking this aggregates and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the purpose protein. In this part, the purpose protein is Defencine1.This is antimicrobial peptide that can inhibit the both gram-positive bacteria. In our experiment, we can use it to inhibit the bacteria in the vase water.
 <!-- Add more about the biology of this part here