Difference between revisions of "Part:BBa K3771010"
Jason Tsai (Talk | contribs) |
|||
Line 3: | Line 3: | ||
<partinfo>BBa_K3771010 short</partinfo> | <partinfo>BBa_K3771010 short</partinfo> | ||
− | to | + | <br><br><b style="font-size:1.3rem">Description |
+ | </b> | ||
+ | <br><br>Outer membrane protein A (OmpA) is a 35 kDa transmembrane protein found in many enterobacteria. It is responsible for maintaining the stability of the bacterial membrane and cell signaling. [1] | ||
+ | <br><br><b style="font-size:1.3rem">Biology | ||
+ | </b> | ||
+ | <br><br>In E. coli, the β-barrel conformation of OmpA is composed of extracellular loops that help play a role in the detection of outer membrane stress and binding of extracellular molecules. OmpA also serves as a porin channel that regulates water transport within the bacterial cell. [2] | ||
+ | <br><br><b style="font-size:1.3rem">Usage | ||
+ | </b> | ||
+ | <br><br>In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein. | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <br> | ||
+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/File:T--NCKU_Tainan--ompA_oprF.gif" style="width:40%;"> | ||
+ | </div> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 07:37, 17 October 2021
Outer Membrane Protein A (OmpA)
Description
Outer membrane protein A (OmpA) is a 35 kDa transmembrane protein found in many enterobacteria. It is responsible for maintaining the stability of the bacterial membrane and cell signaling. [1]
Biology
In E. coli, the β-barrel conformation of OmpA is composed of extracellular loops that help play a role in the detection of outer membrane stress and binding of extracellular molecules. OmpA also serves as a porin channel that regulates water transport within the bacterial cell. [2]
Usage
In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 881
Illegal BamHI site found at 744 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]