Difference between revisions of "Part:BBa K3771011"
Jason Tsai (Talk | contribs) |
Jason Tsai (Talk | contribs) |
||
Line 22: | Line 22: | ||
<br> | <br> | ||
<div style="width=100%; display:flex; align-items: center; justify-content: center;"> | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
− | <img src="https://2021.igem.org/wiki/images/b/b0/T--NCKU_Tainan--pcr_ompA_promoter.jpg" style="width: | + | <img src="https://2021.igem.org/wiki/images/b/b0/T--NCKU_Tainan--pcr_ompA_promoter.jpg" style="width:40%;"> |
</div> | </div> | ||
<p align="center">Fig. 1 Confirmation of PompA fragment by PCR. M: Marker; Lane 1: PompA (~309 bp)</p> | <p align="center">Fig. 1 Confirmation of PompA fragment by PCR. M: Marker; Lane 1: PompA (~309 bp)</p> |
Revision as of 07:28, 17 October 2021
ompA promoter
Description
ompA promoter is a constitutive promoter that drives the expression of OmpA transmembrane protein in E. coli and other enterobacteria [1].
Biology
The ompA promoter is -35/-10 region upstream of the OmpA gene in E. coli MG1655. OmpA protein is regulated by two overlapping promoters ompAp1 and ompAp2 [1,2].
Usage
ompA promoter was used to regulate the expression of OmpA/OprF chimeric protein in the IFN-γ sensing system.
Characterization
The ompA promoter sequence was amplified from E. coli MG1655 and cloned into our plasmid. Agarose gel electrophoresis results confirming the construction with the ompA promoter are shown in Figure 2.
Fig. 1 Confirmation of PompA fragment by PCR. M: Marker; Lane 1: PompA (~309 bp)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]