Difference between revisions of "Part:BBa K3771008"
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<br><b style="font-size:1.3rem">References | <br><b style="font-size:1.3rem">References | ||
</b> | </b> | ||
− | + | <br>[1]Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093 | |
+ | https://pubmed.ncbi.nlm.nih.gov/30516051/ | ||
+ | <br>[2]https://www.uniprot.org/uniprot/Q9Y600 | ||
Revision as of 07:20, 17 October 2021
CSAD-6xHis
Description
CoaBC is an enzyme that weighs 55.4 kDa. CoaBC functions in the JJU10-CoaBC taurine biosynthesis pathway, converting L-cystate to taurine. [3]
Biology
<img src="Biology image" style="width:35%;">
CoaBC is an enzyme in the JJU10-CoaBC pathway, one of three possible taurine synthesis pathways. Its main function is to convert L-cystate to taurine.
Usage
CoaBC was used in vitro testing of taurine production. The sequence for CoaBC and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). A 6xHis-tag is added to the C-terminal of the CoaBC protein, allowing for confirmation of JJU10 expression by western blot using the anti-6xHis antibody.
Characterization
The CoaBC fragment was synthesized by IDT and amplified by PCR. Agarose gel electrophoresis result is shown in Fig. 2.
References
[1]Joo Y-C, Ko YJ, You SK, et al. Creating a New Pathway in Corynebacterium glutamicum for the Production of Taurine as a Food Additive. Journal of Agricultural and Food Chemistry. 2018;66(51):13454-13463. doi:10.1021/acs.jafc.8b05093
https://pubmed.ncbi.nlm.nih.gov/30516051/
[2]https://www.uniprot.org/uniprot/Q9Y600
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 250
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 35