Difference between revisions of "Part:BBa K3771018"

 
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<partinfo>BBa_K3771018 short</partinfo>
 
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<br><b style="font-size:1.3rem">Description</b>
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<br>This composite part is part of a plasmid used in the IFN-γ induction assay to test the function of the IFN-γ sensing system. It consists of the ompA/oprF gene regulated by the ompA promoter (PompA-ompA/oprF) and the orange fluorescent protein (OFP) gene regulated by the pspA promoter (PpspA-ofp).<br>
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<br><b style="font-size:1.3rem">Biology</b>
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<div style="width=100%; display:flex; align-items: center; justify-content: center;">
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<img src="https://2021.igem.org/wiki/images/a/af/T--NCKU_Tainan--ompA_oprF.gif" style="width:35%;">
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<p align="center">Figure. 1 Structure of OmpA/OprF chimeric protein</p>
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Revision as of 06:39, 17 October 2021


PpspA-OFP-PompA-OmpA/OprF


Description


This composite part is part of a plasmid used in the IFN-γ induction assay to test the function of the IFN-γ sensing system. It consists of the ompA/oprF gene regulated by the ompA promoter (PompA-ompA/oprF) and the orange fluorescent protein (OFP) gene regulated by the pspA promoter (PpspA-ofp).


Biology

<img src="T--NCKU_Tainan--ompA_oprF.gif" style="width:35%;">

Figure. 1 Structure of OmpA/OprF chimeric protein


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
    Illegal BamHI site found at 2158
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]