Difference between revisions of "Part:BBa K4090000"
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<partinfo>BBa_K4090000 short</partinfo> | <partinfo>BBa_K4090000 short</partinfo> | ||
− | ice nucleation protein(INP) is a kind of transmembrane protein, which can help proteins to play their role outside the | + | ice nucleation protein(INP) is a kind of transmembrane protein, which can help proteins to play their role outside the membrane. |
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− | === | + | ==Data== |
+ | [[Image:T--SDSZ_China--result_1-2.png|700px|thumb|center|Fig.1-1]] | ||
+ | According to Fig.1-1, all the fragments designed have been successfully made, including S1&S2, which are Silicatein combined with INP, and P1&P2, which are S010_pET28b-csgA-linker-mfp5-7His(pET). | ||
+ | [[Image:T--SDSZ_China--result_1-4.png|700px|thumb|center|Fig.1-2]] | ||
+ | Fig.1-2 represents the results of gel electrophoresis to test the fragment after Gibson Assembly. The length is about 600bp, which, together with the results from sequencing, indicates a positive outcome. | ||
+ | [[Image:T--SDSZ_China--result_1-6.png|700px|thumb|center|Fig.1-3]] | ||
+ | Fig.1-3 represents qualitative OD values, showing that there is sufficient amount of silicatein produced, since: | ||
+ | •Both NC Control are darker (NC1 remain as yellow which indicates that it needs MORE NaOH to turn blue, it contains more acids). | ||
+ | •Both silicatein mixture (with / without homogenization) have higher OD810nm, meaning that there are more bacteria inside the test tube (the solution is thicker / not as clear as NCs). | ||
+ | [[Image:T--SDSZ_China--result_1-7.png|700px|thumb|center|Fig.1-4]] | ||
+ | Fig.1-4 shows the results from TEM testing. Dark proportions represent silicon dioxide produced on the surface of cells. | ||
+ | [[Image:T--SDSZ_China--result_1-3-2.png|700px|thumb|center|Fig.1-5]] | ||
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<partinfo>BBa_K4090000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4090000 SequenceAndFeatures</partinfo> | ||
+ | ==References== | ||
+ | [1]ZHU T, PAULO C, MERROUN, M L, et al. Potential application of biomineralization by Synechococcus PCC8806 for concrete restoration[J]. Sedimentology, 2013,61(1):1-21 | ||
+ | [2]Lechner, Carolin C., and Christian FW Becker. "A sequence‐function analysis of the silica precipitating silaffin R5 peptide." Journal of Peptide Science 20.2 (2014): 152-158. | ||
+ | [3]Poulsen, Nicole, and Nils Kroger. "Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana." Journal of Biological Chemistry 279.41 (2004): 42993-42999. | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display |
Revision as of 04:22, 17 October 2021
Ice Nucleation Protein(INP)
ice nucleation protein(INP) is a kind of transmembrane protein, which can help proteins to play their role outside the membrane.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 321
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 63
Illegal NgoMIV site found at 396
Illegal AgeI site found at 498 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 229
References
[1]ZHU T, PAULO C, MERROUN, M L, et al. Potential application of biomineralization by Synechococcus PCC8806 for concrete restoration[J]. Sedimentology, 2013,61(1):1-21 [2]Lechner, Carolin C., and Christian FW Becker. "A sequence‐function analysis of the silica precipitating silaffin R5 peptide." Journal of Peptide Science 20.2 (2014): 152-158. [3]Poulsen, Nicole, and Nils Kroger. "Silica morphogenesis by alternative processing of silaffins in the diatom Thalassiosira pseudonana." Journal of Biological Chemistry 279.41 (2004): 42993-42999.