Difference between revisions of "Part:BBa K3791007"

Revision as of 01:35, 17 October 2021

gRNA Erythromycin

Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (BBa_K2927006) and a spacer sequence which is specific for the desired gene to target (BBa_K3791002).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3791007 short</partinfo>
-Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2927006 BBa_K2927006]) and a spacer sequence which is specific for the desired gene to target ([https://parts.igem.org/Part:BBa_K3791002 BBa_K3791002]).
+Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (<html><a href="https://parts.igem.org/Part:BBa_K2927006" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K2927006</a></html>) and a spacer sequence which is specific for the desired gene to target (<html><a href="https://parts.igem.org/Part:BBa_K3791002" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791002</a></html>).
 <!-- Add more about the biology of this part here