Difference between revisions of "Part:BBa K3753012:Design"

 
(→‎References)
 
(One intermediate revision by one other user not shown)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
UAS<sub>CIT</sub>-pTEF is synthesized by fusing three UAS<sub>CIT</sub> tandem to the <em>tef2</em> promoter (BBa_K3753003). UAS<sub>CIT</sub> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The amplified product is a 275bp sequence upstream of the <em>cit2</em> promoter start codon.
+
UAS<sub>CIT</sub>-pTEF is synthesized by fusing three UAS<sub>CIT</sub> tandem to the <em>tef2</em> promoter (<partinfo>BBa_K3753003</partinfo>). UAS<sub>CIT</sub> was obtained by PCR amplification using <em>Saccharomyces cerevisiae</em> BY4741 genome as template. The amplified product is a 275bp sequence upstream of the <em>cit2</em> promoter start codon.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 16: Line 14:
  
 
===References===
 
===References===
 +
John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.
 +
 +
Mark Rosenkrantz et al. Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1[J]. Current Genetics, 1994, 25(3) : 185-195.

Latest revision as of 16:19, 16 October 2021


UASCIT-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 845
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 845
    Illegal NgoMIV site found at 1367
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UASCIT-pTEF is synthesized by fusing three UASCIT tandem to the tef2 promoter (BBa_K3753003). UASCIT was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 275bp sequence upstream of the cit2 promoter start codon.

Source

Saccharomyces cerevisiae

References

John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.

Mark Rosenkrantz et al. Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1[J]. Current Genetics, 1994, 25(3) : 185-195.