Difference between revisions of "Part:BBa K3989001"
| Line 3: | Line 3: | ||
<partinfo>BBa_K3989001 short</partinfo> | <partinfo>BBa_K3989001 short</partinfo> | ||
| − | uTEV3 is a | + | uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>. This variant is generated by directed evolution in yeast. |
| − | + | ||
| − | === | + | ===Properties=== |
| + | <html> | ||
| + | The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1. | ||
| + | |||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/b/b6/21_UZurich_uTEV3_efficiency.jpeg"> | ||
| + | <figcaption><b>Fig.1 </b> Comparison of the catalytic efficiency between uTEV3 and the original</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
| + | |||
<!-- --> | <!-- --> | ||
Revision as of 15:46, 16 October 2021
uTEV3, efficiency-enhanced TEV protease variant
uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>. This variant is generated by directed evolution in yeast.
Properties
The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
1) Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.