Difference between revisions of "Part:BBa K4034004"
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This part encodes the Thymidylate synthase (TS). It is codon optimised for <i>E. coli</i> BL21.
 
This part encodes the Thymidylate synthase (TS). It is codon optimised for <i>E. coli</i> BL21.

Revision as of 15:44, 16 October 2021


This part encodes the Thymidylate synthase (TS). It is codon optimised for E. coli BL21.



Description


Thymidylate synthase (TS), has the ability to bind with cellular RNA, forming a complex, which results in translational repression. In general, TS plays an important role in the regulation of the cell cycle, translation, chemosensitivity and apoptosis. Lastly, TS catalyzes the reduction of deoxyuridylate (dUMP) to thymidylate (dTMP), the only de novo method of dTMP production.

Protein Structure


A three secondary stem - loop structure is observed, and this exact structure is hypothesized to influence the TS mRNA translation. With deletion of any one of these repeated sequences, the translational TS mRNA efficiency was altered. The secondary structure of TSase is also important for protein recognition.

Source of this part


NCBI code: AAC75866.1

REFERENCES


Liu J, Schmitz JC, Lin X, Tai N, Yan W, Farrell M, Bailly M, Chen Tm, Chu E. Thymidylate synthase as a translational regulator of cellular gene expression. Biochim Biophys Acta. 2002 Jul 18;1587(2-3):174-82. doi: 10.1016/s0925-4439(02)00080-7. PMID: 12084459.