Difference between revisions of "Part:BBa K3885221"
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In the presence of a strong promoter J23119, the gRNA could be expressed. | In the presence of a strong promoter J23119, the gRNA could be expressed. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | In the presence of a strong promoter J23119,the gRNA could be expressed.In this scientific research project,the gRNA interacts with genetic circuits to achieve the goal of detecting specific biomarker. | ||
+ | |||
+ | ===Characterization=== | ||
+ | CRISPR-Cas9 is a powerful DNA editing tool.A gRNA directs Cas9 to cleave any DNA sequence with a PAM.The gRNA is an important part of the CRISPR system.We have engineered gRNA so that its secondary structure has been transformed. | ||
+ | |||
+ | ===References=== | ||
+ | [1]Moreb E A,Lynch M D. Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity.[J]. Nature communications,2021,12(1):<br> | ||
+ | [2]Hassan Md Mahmudul,Zhang Yingxiao,Yuan Guoliang,De Kuntal,Chen JinGui,Muchero Wellington,Tuskan Gerald A,Qi Yiping,Yang Xiaohan. Construct design for CRISPR/Cas-based genome editing in plants.[J]. Trends in plant science,2021:<br> | ||
+ | [3]He Chao,Liu Hao,Chen Dijun,Xie WenZhao,Wang Mengxin,Li Yuqi,Gong Xin,Yan Wenhao,Chen LingLing. CRISPR-Cereal: A Guide RNA Design Tool Integrating Regulome and Genomic Variation for Wheat, Maize and Rice.[J]. Plant biotechnology journal,2021:<br> | ||
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Latest revision as of 14:38, 16 October 2021
J23119-gRNA
In the presence of a strong promoter J23119, the gRNA could be expressed.
Usage and Biology
In the presence of a strong promoter J23119,the gRNA could be expressed.In this scientific research project,the gRNA interacts with genetic circuits to achieve the goal of detecting specific biomarker.
Characterization
CRISPR-Cas9 is a powerful DNA editing tool.A gRNA directs Cas9 to cleave any DNA sequence with a PAM.The gRNA is an important part of the CRISPR system.We have engineered gRNA so that its secondary structure has been transformed.
References
[1]Moreb E A,Lynch M D. Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity.[J]. Nature communications,2021,12(1):
[2]Hassan Md Mahmudul,Zhang Yingxiao,Yuan Guoliang,De Kuntal,Chen JinGui,Muchero Wellington,Tuskan Gerald A,Qi Yiping,Yang Xiaohan. Construct design for CRISPR/Cas-based genome editing in plants.[J]. Trends in plant science,2021:
[3]He Chao,Liu Hao,Chen Dijun,Xie WenZhao,Wang Mengxin,Li Yuqi,Gong Xin,Yan Wenhao,Chen LingLing. CRISPR-Cereal: A Guide RNA Design Tool Integrating Regulome and Genomic Variation for Wheat, Maize and Rice.[J]. Plant biotechnology journal,2021:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]