Difference between revisions of "Part:BBa K3885341"

 
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<partinfo>BBa_K3885341 short</partinfo>
 
<partinfo>BBa_K3885341 short</partinfo>
  
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In response to the p70 promoter, the transcription factor &#963; 28 is expressed and activates expression from the p28 promoter
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In response to the P70 promoter, the transcription factor &#963; 28 is expressed and activates expression from the P28 promoter
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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To enhance the expression intensity of each component, we use σ28 as the promoter factor. σ28 is a special transcriptional factor concerning the fliA, which is an alternate sigma factor for the class 3 flagella operons, does not exist in Cell-Free system. To verify the functional availability of σ28, we constructed P70a-σ28-P28-deGFP.
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===Characterization===
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This part is used to express and verify the functionality of σ28, so that σ28 will function normally in a Cell-Free system. Through the electroporation experiment, we conducted intra-bacterial verification. The results showed that the fluorescence results of the group containing the part were significantly different from those of the control group (Figure 1 a/b and Figure 2), which proved that the part can perform the expected function, meaning that σ28 can be normal expressed.
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===Design Page===
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P28 is a stronger promoter than P70a, and P28 requires σ28 as a promoter factor. There is no σ28 in a Cell-Free system, and we need to express σ28 and verify its functionality, so we constructed P70a-σ28-P28-deGFP part.
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===References===
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Larson, M. H., Greenleaf, W. J., Landick, R., and Block, S. M. (2008) Applied force reveals mechanistic and energetic details of transcription termination, Cell 132, 971­982.
  
 
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Revision as of 11:13, 16 October 2021


P70-σ28-P28-deGFP

In response to the P70 promoter, the transcription factor σ 28 is expressed and activates expression from the P28 promoter

Usage and Biology

To enhance the expression intensity of each component, we use σ28 as the promoter factor. σ28 is a special transcriptional factor concerning the fliA, which is an alternate sigma factor for the class 3 flagella operons, does not exist in Cell-Free system. To verify the functional availability of σ28, we constructed P70a-σ28-P28-deGFP.

Characterization

This part is used to express and verify the functionality of σ28, so that σ28 will function normally in a Cell-Free system. Through the electroporation experiment, we conducted intra-bacterial verification. The results showed that the fluorescence results of the group containing the part were significantly different from those of the control group (Figure 1 a/b and Figure 2), which proved that the part can perform the expected function, meaning that σ28 can be normal expressed.

Design Page

P28 is a stronger promoter than P70a, and P28 requires σ28 as a promoter factor. There is no σ28 in a Cell-Free system, and we need to express σ28 and verify its functionality, so we constructed P70a-σ28-P28-deGFP part.

References

Larson, M. H., Greenleaf, W. J., Landick, R., and Block, S. M. (2008) Applied force reveals mechanistic and energetic details of transcription termination, Cell 132, 971­982.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 487
    Illegal SapI.rc site found at 512
    Illegal SapI.rc site found at 668