Difference between revisions of "Part:BBa K3989002"

(→‎Structure and binding site)
Line 13: Line 13:
 
</figure>
 
</figure>
 
<html>
 
<html>
−
 
−
<!-- -->
 
−
<span class='h3bb'>Sequence and Features</span>
 
−
<partinfo>BBa_K3989002 SequenceAndFeatures</partinfo>
 
−
 
−
 
−
<!-- Uncomment this to enable Functional Parameter display
 
−
===Functional Parameters===
 
−
<partinfo>BBa_K3989002 parameters</partinfo>
 
−
<!-- -->
 
  
 
===Reference===
 
===Reference===

Revision as of 09:41, 16 October 2021


eTEV, efficiency-enhanced TEV protease variant

eTEV protease is mutant from the original TEV protease. Seven amino acids(S3I, P8Q, S31T, E79G, T173A, V219R, A231V) are mutated in this variant and the catalytic efficiency is 2-fold higher than the original one.


Structure and binding site

Fig. 1 . - Sub-structure at the binding site of eTEV.
===Reference=== 1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.