Difference between revisions of "Part:BBa K3776019:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3776022 short</partinfo>
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<partinfo>BBa_K3776019 short</partinfo>
  
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<partinfo>BBa_K3776022 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3776019 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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composite test
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This CRISPR-RNA integrase complex was devised by Vo et al. (2020).
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===Source===
 
===Source===
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===References===
 
===References===
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[1]Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H. (2020). CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering. Nature Biotechnology, 39(4), 480–489. https://doi.org/10.1038/s41587-020-00745-y

Latest revision as of 09:03, 16 October 2021


INTEGRATE system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1578
    Illegal NheI site found at 3665
    Illegal NheI site found at 7783
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1004
    Illegal BglII site found at 1795
    Illegal BglII site found at 2325
    Illegal BglII site found at 7570
    Illegal BamHI site found at 147
    Illegal BamHI site found at 8698
    Illegal XhoI site found at 8681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7702
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 113
    Illegal BsaI.rc site found at 87
    Illegal SapI.rc site found at 3081


Design Notes

This CRISPR-RNA integrase complex was devised by Vo et al. (2020).

Source

composite test

References

[1]Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H. (2020). CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering. Nature Biotechnology, 39(4), 480–489. https://doi.org/10.1038/s41587-020-00745-y