Difference between revisions of "Part:BBa K3868073"
Line 3: Line 3:
 
<partinfo>BBa_K3868073 short</partinfo>
 
<partinfo>BBa_K3868073 short</partinfo>
  
−
Gene from S. pyogenes for the pCas plasmid of the Crispr-Cas9 system,plays a role in cutting DNA double strands.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pCas plasmid.
+
Gene from pYLXP’-T7Nluc encoding Nluc protein, can detect promoter intensity with luciferase.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP’- Promoter-Nluc plasmid.
−
 
+
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:23, 16 October 2021


Nluc

Gene from pYLXP’-T7Nluc encoding Nluc protein, can detect promoter intensity with luciferase.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP’- Promoter-Nluc plasmid. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
  • 1000
    COMPATIBLE WITH RFC[1000]