Difference between revisions of "Part:BBa K3796217"
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<partinfo>BBa_K3796217 short</partinfo> | <partinfo>BBa_K3796217 short</partinfo> | ||
| − | + | This is the gene circuit that can be used to verify if the toxin gene, <i>ndoA</i>, works as expected. <i>ndoA</i> encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system in <i>Bacillus subtilis</i>. We use this gene circuit to verify if <i>ndoA</i> is toxic for the growth of <i>C. glutamicum</i> as well, by comparing its growth state with/without induction. | |
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Revision as of 03:05, 16 October 2021
Ptac-rbs-ndoA-terminator
This is the gene circuit that can be used to verify if the toxin gene, ndoA, works as expected. ndoA encodes endoribonuclease EndoA, a toxic component of a type II toxin-antitoxin (TA) system in Bacillus subtilis. We use this gene circuit to verify if ndoA is toxic for the growth of C. glutamicum as well, by comparing its growth state with/without induction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]