Difference between revisions of "Part:BBa K3853011"

Revision as of 18:37, 15 October 2021

dCas9-SpyCatcher

This part consists of defective endonuclease dCas9(BBa_K3853003), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use BBa_K3853055 to construct the expression system and to express and to purify the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3576
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3960
Illegal AgeI site found at 3304
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3853011 short</partinfo>
-This part consists of defective endonuclease dCas9(<partinfo>BBa_K3853003</partinfo>), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use <partinfo>BBa_3853055</partinfo> to construct the expression system and to express and to purify the protein.
+This part consists of defective endonuclease dCas9(<partinfo>BBa_K3853003</partinfo>), modified immunoglobulin-like domain SpyCatcher and Ser/Gly linker(S/G linker). S/G linker is applied to link dCas9 with SpyCatcher. dCas9 can specifically target and bind dsDNA under the mediation of single-guide RNA (sgRNA), meanwhile, SpyCatcher is usually used for protein ligation. dCas9-SpyCatcher has been reported as connection system to assemble protein-nucleic-acid complex. We use <partinfo>BBa_K3853055</partinfo> to construct the expression system and to express and to purify the protein.
 <!-- Add more about the biology of this part here