Difference between revisions of "Part:BBa K3788004:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.
+
<p>After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.</p>
−
 
+
  
  
 
===Source===
 
===Source===
  
−
The 6His_P20__Flag_cry11Aa__Strep_cyt1Aa sequence is made from BBa_K3788002, BBa_K3788001 (BBa_K3788003) and BBa_K3788000 parts.  
+
<p> The 6His_P20__Flag_cry11Aa__Strep_cyt1Aa sequence is made from BBa_K3788002, BBa_K3788001 (BBa_K3788003) and BBa_K3788000 parts. </p>
−
Theses 2 parts are optimised sequences for E. coli obtain from Part:BBa_K2938008, Part:BBa_K2938005 and Part:BBa_K2938003.
+
<p>Theses 2 parts are optimised sequences for E. coli obtain from Part:BBa_K2938008, Part:BBa_K2938005 and Part:BBa_K2938003.</p>
  
  
 
===References===
 
===References===
 +
<p> - Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).</p>
 +
<p> - Hua, G., Masson, L., Jurat-Fuentes, J. L., Schwab, G. & Adang, M. J. Binding Analysis of Bacillus thuringiensis Cry δ-Endotoxins Using Brush Border Membrane Vesicles of  Ostrinia nubilalis. Appl. Environ. Microbiol. 67, 872–879 (2001). </p>
 +
<p>- Manasherob, R. et al. Effect of Accessory Proteins P19 and P20 on Cytolytic Activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli. Curr. Microbiol. 43, 355–364 (2001).

Revision as of 15:48, 15 October 2021


6His-P20, Flag-Cry11Aa and Strep-Cyt1Aa coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
    Illegal XhoI site found at 2438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After the sequence eventual mutation was prevented : Amino acids have been changed to avoid the formation of a stop codon and also to remove restriction sites corresponding to RFC10 and RFC25.


Source

The 6His_P20__Flag_cry11Aa__Strep_cyt1Aa sequence is made from BBa_K3788002, BBa_K3788001 (BBa_K3788003) and BBa_K3788000 parts.

Theses 2 parts are optimised sequences for E. coli obtain from Part:BBa_K2938008, Part:BBa_K2938005 and Part:BBa_K2938003.


References

- Bravo, A., Gill, S. S. & Soberón, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 49, 423–435 (2007).

- Hua, G., Masson, L., Jurat-Fuentes, J. L., Schwab, G. & Adang, M. J. Binding Analysis of Bacillus thuringiensis Cry δ-Endotoxins Using Brush Border Membrane Vesicles of Ostrinia nubilalis. Appl. Environ. Microbiol. 67, 872–879 (2001).

- Manasherob, R. et al. Effect of Accessory Proteins P19 and P20 on Cytolytic Activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli. Curr. Microbiol. 43, 355–364 (2001).