Difference between revisions of "Part:BBa K3788001:Experience"
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===Applications of BBa_K3788001=== | ===Applications of BBa_K3788001=== | ||
| − | <h2> PCR amplification of flag<i>Cry11Aa</i> gene : </ | + | <h2> PCR amplification of flag<i>Cry11Aa</i> gene : </h2> |
<p> The DNA sequence of flag<i>cry11Aa</i> was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>1956bp </b> and a 2000bp band is observed. Amplification of DNAs sequences with primers were validated.</p> | <p> The DNA sequence of flag<i>cry11Aa</i> was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>1956bp </b> and a 2000bp band is observed. Amplification of DNAs sequences with primers were validated.</p> | ||
Revision as of 14:44, 15 October 2021
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Applications of BBa_K3788001
PCR amplification of flagCry11Aa gene :
The DNA sequence of flagcry11Aa was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 1956bp and a 2000bp band is observed. Amplification of DNAs sequences with primers were validated.
Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 pB), flagcry11Aa (expected 1847 pB), strepcyt1Aa (expected 775 pB) and 6hisp20 (expected 568 pB); L (SMART Ladder, its size is shown on the right side).
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