Difference between revisions of "Part:BBa K3788000:Experience"
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===Applications of BBa_K3788000=== | ===Applications of BBa_K3788000=== | ||
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| + | <h2> PCR amplification of strep<i>cyt1Aa</i> gene : </h2> | ||
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| + | <p> The DNA sequence of strep<i>cyt1Aa</i> was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>775 pb</b> and a 800pb band is observed. Amplification of DNAs sequences with primers were validated.</p> | ||
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| + | https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png | ||
| + | <p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i> | ||
| + | a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 pB), flagcry11Aa (expected 1847 pB), strepcyt1Aa (expected 775 pB) and 6hisp20 (expected 568 pB); L (SMART Ladder, its size is shown on the right side). </i></p> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 13:20, 15 October 2021
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Applications of BBa_K3788000
PCR amplification of strepcyt1Aa gene :
The DNA sequence of strepcyt1Aa was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 775 pb and a 800pb band is observed. Amplification of DNAs sequences with primers were validated.
Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 pB), flagcry11Aa (expected 1847 pB), strepcyt1Aa (expected 775 pB) and 6hisp20 (expected 568 pB); L (SMART Ladder, its size is shown on the right side).
User Reviews
UNIQeb135b92518a074d-partinfo-00000000-QINU UNIQeb135b92518a074d-partinfo-00000001-QINU