Difference between revisions of "Part:BBa K3788000"
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<p> BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxins have a strep tag II in Nter to allow to purified the protein Cyt1Aa and allow the detection and specific identification of Cyt1Aa by WesterBlot. The gene cyt1Aa was optimised for E. coli K12. </p>
 
<p> BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxins have a strep tag II in Nter to allow to purified the protein Cyt1Aa and allow the detection and specific identification of Cyt1Aa by WesterBlot. The gene cyt1Aa was optimised for E. coli K12. </p>
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<p></p>
  
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<p><u> Expected size </u>:</p>
 
<p> strepII_<i>cyt1Aa</i> gene is 777pB </p>  
 
<p> strepII_<i>cyt1Aa</i> gene is 777pB </p>  
 
<p> Strep_Cyt1Aa protein is 28,5 kDa </p>  
 
<p> Strep_Cyt1Aa protein is 28,5 kDa </p>  
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<p> Cyt1Aa would facilitate the binding of Cry11Aa to the BBMV receptor, by interaction of the loop α8, β4 and loop 2 regions of Cry11Aa at the level of the loop β6 – αE and part of β7 of Cyt1Aa. This interaction allows easy insertion of Cry11Aa into membranes. </p>  
 
<p> Cyt1Aa would facilitate the binding of Cry11Aa to the BBMV receptor, by interaction of the loop α8, β4 and loop 2 regions of Cry11Aa at the level of the loop β6 – αE and part of β7 of Cyt1Aa. This interaction allows easy insertion of Cry11Aa into membranes. </p>  
  
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Revision as of 13:06, 15 October 2021


StrepII-Cyt1Aa

BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxins have a strep tag II in Nter to allow to purified the protein Cyt1Aa and allow the detection and specific identification of Cyt1Aa by WesterBlot. The gene cyt1Aa was optimised for E. coli K12.

Expected size :

strepII_cyt1Aa gene is 777pB

Strep_Cyt1Aa protein is 28,5 kDa


Mode of action of Cyt toxins:

Cyt (Cytolitic) toxins are produced in pro-toxin form, they will undergo proteolytic cleavage in N-ter and C-ter allowing the release of the active form of Cyt toxins. The mature forms will interact with the membrane lipids of the midgut cells and will insert themselves into the membrane, forming β-barrels. Cyt toxin are different in them domains.

Cyt1Aa would facilitate the binding of Cry11Aa to the BBMV receptor, by interaction of the loop α8, β4 and loop 2 regions of Cry11Aa at the level of the loop β6 – αE and part of β7 of Cyt1Aa. This interaction allows easy insertion of Cry11Aa into membranes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]