Difference between revisions of "Part:BBa K4081994"

Revision as of 03:19, 15 October 2021

GAP promoter

Generally, GAP promoter can drive the expression of glyceraldehyde-3-phosphate dehydrogenase gene in many species.

Biology and Usage

A lot of attention has been drawn to Glyceraldehyde-3-Phosphate Dehydrogenase (GAP) promoter. GAP mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that it can be effectively used in the high-efficiency expression of transgenes[1]. In our project, we use GAP promoter to promote the expression of FKP. General vectors have been designed to rely on homologous GAPDH promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-===Usage and Biology===
 A lot of attention has been drawn to Glyceraldehyde-3-Phosphate Dehydrogenase (GAP) promoter. GAP mRNA accounts for 2–5% of the poly(A) RNA in yeasts, suggesting that it can be effectively used in the high-efficiency expression of transgenes[1]. In our project, we use GAP promoter to promote the expression of FKP. General vectors have been designed to rely on homologous GAPDH promoter regions to drive the expressions of heterologous genes in yeasts and other filamentous fungi.