Difference between revisions of "Part:BBa K3822005"

 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3822005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3822005 SequenceAndFeatures</partinfo>
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===Result===
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==1.Construction of the plasmids==
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The miRNA “ sponge” method was introduced by several years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. More effective are sponges containing bulged sites that are mispaired opposite miRNA positions 9-12 (Ebert et al. 2007, Gentner et al. 2009), presumably because they form a more stable interaction with the miRNA. pEGFP-miR196a-148a 2.0(Part K3822005) was designed based on the principle of effective sponges containing bulged sites (Fig. 1). We compare pEGFP-miR196a-148a 2.0 with pEGFP-miR196a-148a 1.0 (Part K3160007,designed by a 2019 iGEM team) on monitoring the expression of miRNAs in gastric cancer cells.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3822005 parameters</partinfo>
 
<partinfo>BBa_K3822005 parameters</partinfo>
 
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Revision as of 01:55, 15 October 2021


miR196a-148a 2.0

miR196a-148a 2.0 is an improvement of Part BBa_K3160007. which could monitor the expression of miRNAs in gastric cancer cells

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Result

1.Construction of the plasmids

The miRNA “ sponge” method was introduced by several years ago as a means to create continuous miRNA loss of function in cell lines and transgenic organisms. More effective are sponges containing bulged sites that are mispaired opposite miRNA positions 9-12 (Ebert et al. 2007, Gentner et al. 2009), presumably because they form a more stable interaction with the miRNA. pEGFP-miR196a-148a 2.0(Part K3822005) was designed based on the principle of effective sponges containing bulged sites (Fig. 1). We compare pEGFP-miR196a-148a 2.0 with pEGFP-miR196a-148a 1.0 (Part K3160007,designed by a 2019 iGEM team) on monitoring the expression of miRNAs in gastric cancer cells.