Difference between revisions of "Part:BBa K3970028"
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<partinfo>BBa_K3970028 short</partinfo> | <partinfo>BBa_K3970028 short</partinfo> | ||
| − | This part is the effector of the upstream regulator, the product of the LexA will bind to the following LexO part and active the downstream cpcE gene expression.With the regulation of cpcE,the lyase a-subunit will not effect the growth of yeast. | + | ==Usage and Biology== |
| + | This part is the effector of the upstream regulator, the product of the LexA will bind to the following LexO part and active the downstream cpcE gene expression. With the regulation of cpcE, the lyase a-subunit will not effect the growth of yeast. | ||
| + | |||
| + | ==Construction== | ||
| + | [[File:0028-1.png|600px|thumb|center]] | ||
| + | |||
| + | ==Sequence== | ||
| + | <p style="word-wrap:break-word;">CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAAGGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACGGTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGTTTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCATCCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTTGGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGAtggtccgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc</p> | ||
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Latest revision as of 14:46, 14 October 2021
LexO-cpTDH3-CpcE-tCYC1
Usage and Biology
This part is the effector of the upstream regulator, the product of the LexA will bind to the following LexO part and active the downstream cpcE gene expression. With the regulation of cpcE, the lyase a-subunit will not effect the growth of yeast.
Construction
Sequence
CTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGCTGTATATATATACAGATGGTTAGAGTTGCTATTAACGGTTTCGGTAGAATCGGTAGATTGGTCATGAGAATTGCTTTGTCTAGACCAAACGTCGAAGTTGTTGCTTTGAACGACCCATTCATCACCAACGACTACGCTGCTTACATGTTCAAGTACGACTCCACTCACGGTAGATACGCTGGTGAAGTTTCCCACGATGACAAGCACATCATTGTCGATGGTAAGAAGATTGCTACTTACCAAGAAAGAGACCCAGCTAACTTGCCATGGGGTTCTTCCAACGTTGACATCGCCATTGACTCCACTGGTGTTTTCAAGGAATTAGACACTGCTCAAAAGCACATTGACGCTGGTGCCAAGAAGGTTGTTATCACTGCTCCATCTTCCACCGCCCCAATGTTCGTCATGGGTGTTAACGAAGAAAAATACACTTCTGACTTGAAGATTGTTTCCAACGCTTCTTGTACCACCAACTGTTTGGCTCCATTGGCCAAGGTTATCAACGATGCTTTCGGTATTGAAGAAGGTTTGATGACCACTGTCCACTCTTTGACTGCTACTCAAAAGACTGTTGACGGTCCATCCCACAAGGACTGGAGAGGTGGTAGAACCGCTTCCGGTAACATCATCCCATCCTCCACCGGTGCTGCTAAGGCTGTCGGTAAGGTCTTGCCAGAATTGCAAGGTAAGTTGACCGGTATGGCTTTCAGAGTCCCAACCGTCGATGTCTCCGTTGTTGACTTGACTGTCAAGTTGAACAAGGAAACCACCTACGATGAAATCAAGAAGGTTGTTAAGGCTGCCGCTGAAGGTAAGTTGAAGGGTGTTTTGGGTTACACCGAAGACGCTGTTGTCTCCTCTGACTTCTTGGGTGACTCTCACTCTTCCATCTTCGATGCTTCCGCTGGTATCCAATTGTCTCCAAAGTTCGTCAAGTTGGTCTCCTGGTACGACAACGAATACGGTTACTCTACCAGAGTTGTCGACTTGGTTGAACACGTTGCCAAGGCTTAAATGTTGTCATTAGAACAATTGGCTACCCAATTGGAATCTCCAAACTCTAGAGACAGATTGTTGGCCCTTGCTGCCTTAAGAGATGTTCCACCTCCAGAAGCTGTTCCATTGATCAAGAAGGTTTTGTGGGATGACAATTTGCAAATCCGTTCCATGGCTGTCTTTGCTTTGGGTGTTAAGCCTACCCCAGAATGTTTCGACATTTTAGTCCAATTGTTGGAAACTGAACCAGACTACGGTATCAGAGCTGATGCTGCTGGTGCCTTGGGTTATTTGCAAGACCAAAGAGCCTTCGCTCCATTAGTCAGAGCTTTCTACGAAGACACTGATTGGTTGGTTAGATTCTCTGCCGCTGTTTCCTTAGGTAACTTGAAAGATGTCCGTGCTGTCGAAGTCTTGAGATCTGCTTTGTCCTCTAACGAGGTTGTTATGTTGCAAGGTGCTATTGCTGCTTTGGGTGAAATTGGTGACCCATCCATTGTTGAAGAAATCTTGCCATTCGTCACTGCTGAAGACTGGTTGATTAGACAAAGATTAGCCGAAGCCCTAGGTAACTTGCCATCTCCAAAGACCCAATCCGCACTAGAATACTTGGCTAAGGATGAACACCCACACGTTGCTGCCGCTGCTGAAATCTCTTTGCAACGTTTGGCTCAAAGATGAtggtccgagtcatgtaattagttatgtcacgcttacGttcacgccctccccccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaaggctttaatttgc
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]