Difference between revisions of "Part:BBa K3927017"
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===Design=== | ===Design=== | ||
| + | This part was derived from the gene encoding for Human Beta Defensin 2 (DEFB4). The part sequence has been codon optimized for expression in S. cerevisiae and P. pastoris. | ||
===Characterization=== | ===Characterization=== | ||
Revision as of 12:43, 14 October 2021
Human Beta Defensin 2 (S. cerevisiae)
Coding sequence for the human immune protein, human beta defensin 2. Codon optimized for S. cerevisiae).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Description
This part contains the CDS for Human Beta Defensin 2 (HBD2) with a 6xHis-tag attached to the C-terminus of the peptide sequence.
Usage
This part contains only the CDS and 6xHis-tag. Stop codon is required at the end of the sequence as it is not included.
Promoter and terminator is required for successful expression, additional secretion signal peptide may be required to successfully express and purify HBD2 from culture.
Design
This part was derived from the gene encoding for Human Beta Defensin 2 (DEFB4). The part sequence has been codon optimized for expression in S. cerevisiae and P. pastoris.
Characterization
Part characterization was performed using the composite part BBa_K3927018 (HB2 with mating factor alpha secretion tag). Please refer to that part page for more details.