Difference between revisions of "Part:BBa K313010"
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We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression. | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression. | ||
| − | Group: SYSU-CHINA 2021 | + | <br>Group: SYSU-CHINA 2021 |
| − | Author: Zhao bingnan | + | <br>Author: Zhao bingnan |
| − | Summary: the 2D and 3D sturcture and application of MS2 aptamer | + | <br>Summary: the 2D and 3D sturcture and application of MS2 aptamer |
| − | resource:2018_Book_VirusProteinAndNucleoproteinCo,Organization of Intracellular Reactions with Rationally Designed RNA Assemblies | + | <br>resource:2018_Book_VirusProteinAndNucleoproteinCo,Organization of Intracellular Reactions with Rationally Designed RNA <br>Assemblies |
| − | Uploads: MS2 aptamer on the circRNA scaffold.png;SYSU-CHINA circRNA-1.png;SYSU-CHINA circRNA-2.png;The principle of spite EGFP.png;MS2 binding mechanism.png | + | <br>Uploads: MS2 aptamer on the circRNA scaffold.png;SYSU-CHINA circRNA-1.png;SYSU-CHINA circRNA-2.png;The principle of spite EGFP.png;MS2 binding mechanism.png |
===Applications of BBa_K313010=== | ===Applications of BBa_K313010=== | ||
Revision as of 10:03, 13 October 2021
loading sequence of RNA phage MS2
This sequence is necessary for loading of RNA into the capsid of RNA phage MS2. The coat proteins of phage MS2 is known to aggregate around this sequence.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.
We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
Group: SYSU-CHINA 2021
Author: Zhao bingnan
Summary: the 2D and 3D sturcture and application of MS2 aptamer
resource:2018_Book_VirusProteinAndNucleoproteinCo,Organization of Intracellular Reactions with Rationally Designed RNA
Assemblies
Uploads: MS2 aptamer on the circRNA scaffold.png;SYSU-CHINA circRNA-1.png;SYSU-CHINA circRNA-2.png;The principle of spite EGFP.png;MS2 binding mechanism.png
Applications of BBa_K313010
We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
2D stucture
UNIQd164dc1b7e533690-partinfo-00000001-QINU
UNIQd164dc1b7e533690-partinfo-00000002-QINU
We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:
3D stucture
UNIQd164dc1b7e533690-partinfo-00000003-QINU
UNIQd164dc1b7e533690-partinfo-00000004-QINU
We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:
MS2 binding mechanism
UNIQd164dc1b7e533690-partinfo-00000005-QINU UNIQd164dc1b7e533690-partinfo-00000006-QINU
split EGFP
UNIQd164dc1b7e533690-partinfo-00000007-QINU
UNIQd164dc1b7e533690-partinfo-00000008-QINU
We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.
