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| | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression. | | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression. |
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| − | ===Applications of BBa_K313010===
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| − | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
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| − | ===2D stucture===
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| − | <!-- DON'T DELETE --><partinfo>BBa_K313010 StartReviews</partinfo>
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| − | experimental data
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| − | <br>We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:
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| − | [[Image:MS2_aptamer_on_the_circRNA_scaffold.png | border | center | 300px]]
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| − | <font size="1"><center>Figure 1.2D stucture predicted by RNAfold</center></font><br/>
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| − | [[Image:SYSU-CHINA_circRNA-1.png | border | center | 300px]]
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| − | <font size="1"><center>Figure 2.2D stucture predicted by StructureEditor</center></font><br/>
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| − | ===3D stucture===
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| − | <br>We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:
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| − | <br>[[image:SYSU-CHINA circRNA-2.png |border|center|300px]]
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| − | <br><font size="1"><center>Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab</center></font>
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| − | ===MS2 binding mechanism===
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| − | <br>[[Image:MS2 binding mechanism.png | border | center | 300px]]
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| − | <br><center><font size="1">Figure 4. MS2 binding mechanism</font></center>
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| − | <br>
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| − | ===split EGFP===
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| − | <br>We are SYSU-CHINA,and this year we use this part as MS2 coat protein's aptamer to do a split EGFP experiment,for the proof of concept.Here we found a research article, which use liner RNA with MS2 and PP7 aptamer, to do the split EGFP experiment.Below are their experimental data.
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| − | <br>[[Image:The_principle_of_spite_EGFP.png | border | center | 300px]]
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| − | <br><center><font size="1">Figure 3.the principle and experimental data of split EGFP</font>
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| − | <!-- this is a RBS of MS2
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| − | ===Usage and Biology===
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| − | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
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| − | <!-- -->
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| − | <span class='h3bb'>Sequence and Features</span>
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| − | <partinfo>BBa_K313010 SequenceAndFeatures</partinfo>
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K313010 parameters</partinfo>
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| − | <!-- -->
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| − | __NOTOC__
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| − | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
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| − |
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| − | ===Applications of BBa_K313010===
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| − | We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
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| − | ===2D stucture===
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| − | <!-- DON'T DELETE --><partinfo>BBa_K313010 StartReviews</partinfo>
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| − | <I>Username</I>
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| − | experimental data
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| − | |};
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| − | <!-- End of the user review template -->
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| − | <!-- DON'T DELETE --><partinfo>BBa_K313010 EndReviews</partinfo>
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| − | <br>We use RNAfold and StructureEditor to predict 2D structure of our circRNA, which contains MS2 aptamer.The results are below:
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| − | [[Image:MS2_aptamer_on_the_circRNA_scaffold.png | border | center | 300px]]
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| − | <font size="1"><center>Figure 1.2D stucture predicted by RNAfold</center></font><br/>
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| − | [[Image:SYSU-CHINA_circRNA-1.png | border | center | 300px]]
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| − | <font size="1"><center>Figure 2.2D stucture predicted by StructureEditor</center></font><br/>
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| − | ===3D stucture===
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| − | |};
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| − | <!-- DON'T DELETE --><partinfo>BBa_K313010 EndReviews</partinfo>
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| − | <br>We use this website:http://biophy.hust.edu.cn/new/3dRNA/create, to predict the 3D structure of our circRNA, which contains MS2 aptamer.The result lists below:
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| − | <br>[[image:SYSU-CHINA circRNA-2.png |border|center|300px]]
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| − | <br><font size="1"><center>Figure 3. 3D stucture of MS2 aptamer predicted by Xiaolab</center></font>
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| − |
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| − | ===MS2 binding mechanism===
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| − | <br>[[Image:MS2 binding mechanism.png | border | center | 300px]]
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| − | <br><center><font size="1">Figure 4. MS2 binding mechanism</font></center>
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| − | <br>
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| − | ===split EGFP===
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This sequence is necessary for loading of RNA into the capsid of RNA phage MS2.
The coat proteins of phage MS2 is known to aggregate around this sequence.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_MS2 Phage MS2] assay page.
We are SYSU-CHINA 2021 and this year we use circRNA to colocalize enzymes, which are fused to RNA binding proteins(RBP). We choose MS2 coat protein and PP7 coat protein as our RBP,and their aptamer on circRNA.For contribution, we predict its 2D and 3D structure,to present a visual impression.
