Difference between revisions of "Part:BBa K3971011"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | CscB (sucrose permease) is a sucrose/H+ symporter from E.coli that relies on an H+ gradient across the cell membrane to transport sucrose, and allows bacteria to take up sucrose [1]. The first study of using CscB as a sucrose exporter in cyanobacteria was reported by Ducat et. al [2] where they showed that Synechococcus elongatus 7942 could export sucrose irreversibly to concentrations of >10 mM without culture toxicity. | + | CscB (sucrose permease) is a sucrose/H+ symporter from ''E. coli'' W that relies on an H+ gradient across the cell membrane to transport sucrose, and allows bacteria to take up sucrose [1]. The first study of using CscB as a sucrose exporter in cyanobacteria was reported by Ducat et. al [2] where they showed that Synechococcus elongatus 7942 could export sucrose irreversibly to concentrations of >10 mM without culture toxicity. |
The higher pH value in the extracellular medium creates a reversed proton gradient, which enables the engineered cyanobacteria to export sucrose outside the cell [1]. | The higher pH value in the extracellular medium creates a reversed proton gradient, which enables the engineered cyanobacteria to export sucrose outside the cell [1]. | ||
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| + | [[File:T--IISER-Pune-India--cscoperon.jpg|thumb|300x370px|centre|csc operon in E.coli W for sucrose metabolism. Figure adapted from: Bruschi, Michele, et al. "A transferable sucrose utilization approach for non-sucrose-utilizing Escherichia coli strains." Biotechnology advances 30.5 (2012): 1001-1010.]] | ||
===References=== | ===References=== | ||
| + | 1: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8. | ||
| + | 2: Ducat, Daniel C., et al. "Rerouting carbon flux to enhance photosynthetic productivity." Applied and environmental microbiology 78.8 (2012): 2660-2668. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 11:46, 12 October 2021
cscB (sucrose permease) for sucrose transport.
Usage and Biology
CscB (sucrose permease) is a sucrose/H+ symporter from E. coli W that relies on an H+ gradient across the cell membrane to transport sucrose, and allows bacteria to take up sucrose [1]. The first study of using CscB as a sucrose exporter in cyanobacteria was reported by Ducat et. al [2] where they showed that Synechococcus elongatus 7942 could export sucrose irreversibly to concentrations of >10 mM without culture toxicity.
The higher pH value in the extracellular medium creates a reversed proton gradient, which enables the engineered cyanobacteria to export sucrose outside the cell [1].
References
1: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.
2: Ducat, Daniel C., et al. "Rerouting carbon flux to enhance photosynthetic productivity." Applied and environmental microbiology 78.8 (2012): 2660-2668. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 525