Difference between revisions of "Part:BBa K4040020"

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[[File:T--NMU_China--gp130-tev.jpg|300px|thumb|left|<b>Figure1.</b>The structure of gp130-TEV.]]
 
[[File:T--NMU_China--gp130-tev.jpg|300px|thumb|left|<b>Figure1.</b>The structure of gp130-TEV.]]
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[[File:T--NMU_China--IL-6R.jpg|600px|thumb|right|<b>Figure1.</b>The structure of the composite IL-6R.]]
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[[File:T--NMU_China--IL-6R.jpg|550px|thumb|right|<b>Figure1.</b>The structure and downstream activation of the composite IL-6R.]]
  
 
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Revision as of 11:46, 11 October 2021


Synthetic Receptor GP130-TEV

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. We used CMV as the promoter. When the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed.  Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway.  

Figure1.The structure of gp130-TEV.
Figure1.The structure and downstream activation of the composite IL-6R.