Difference between revisions of "Part:BBa K4040020"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. And when the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed. Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway. | Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. And when the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed. Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway. | ||
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| + | [[File:T--NMU_China--gp130-tev.jpg|400px|thumb|center|<b>Figure1.</b>Vector maps of tested CAR designs and schematics showing the structures of CARs.]] | ||
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Revision as of 11:37, 11 October 2021
Synthetic Receptor GP130-TEV
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1508
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Based on the mechanism of Tango/MESA, we bind TEV-protase to GP130. And when the concentration of IL-6 rises to a certain level, IL-6 binds to mIL-6R (membrane-bound IL-6R), homodimerization of gp130 is induced. A high-affinity functional receptor complex of IL-6, IL-6R and gp130 is formed. Then, TEV-protase connected behind is activated. TEV-protase binds to TCS and releases transcription factors on the other two receptors through enzyme digestion to initiate the subsequent pathway.
