Difference between revisions of "Part:BBa K3896000"
 
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This part is the coding gene of pmrB protein in human cell dipeptidyl peptidase 4 (DPP4) recombinant protein and bacterial PmrA / PmrB two-component system.
 
This part is the coding gene of pmrB protein in human cell dipeptidyl peptidase 4 (DPP4) recombinant protein and bacterial PmrA / PmrB two-component system.
  
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We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original pmrB with the core binding domain of DPP4 protein Gly260 to Asp330 to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase pmrb autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA.
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We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein Gly260 to Asp330 to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA.
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https://static.igem.org/mediawiki/parts/3/30/T--NEU_CHINA--test03.png
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 13:32, 10 October 2021


The coding gene of DPP4-PmrB recombinant protein

This part is the coding gene of pmrB protein in human cell dipeptidyl peptidase 4 (DPP4) recombinant protein and bacterial PmrA / PmrB two-component system.

We replaced the Fe (III) sensitive domains Trp34 to Glu64 of the original PmrB with the core binding domain of DPP4 protein Gly260 to Asp330 to be stimulated by the S protein of MERS-CoV. After detecting the S protein of MERS-CoV, the sensor kinase PmrB autophosphorylates the highly conserved histidine residue, and then transfers the phosphate group to the conserved aspartate residue in its homologous reaction regulator PmrA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1147
  • 1000
    COMPATIBLE WITH RFC[1000]