Difference between revisions of "Part:BBa K3934015"
 
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<partinfo>BBa_K3934015 short</partinfo>
 
<partinfo>BBa_K3934015 short</partinfo>
  
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A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC).  
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A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC) [1].  
  
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K3934015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3934015 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K3934015 parameters</partinfo>
 
<partinfo>BBa_K3934015 parameters</partinfo>
 
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<b>References</b>
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<br>[1]    W. T. Booth et al., ‘Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability’, ACS Omega, vol. 3, no. 1, pp. 760–768, Jan. 2018, doi: 10.1021/acsomega.7b01598.

Latest revision as of 13:15, 10 October 2021


6xHis-Tag for protein purification

A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC) [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] W. T. Booth et al., ‘Impact of an N-terminal Polyhistidine Tag on Protein Thermal Stability’, ACS Omega, vol. 3, no. 1, pp. 760–768, Jan. 2018, doi: 10.1021/acsomega.7b01598.