Difference between revisions of "Part:BBa K3739106"

Revision as of 13:03, 10 October 2021

K525998(T7-RBS)-Aly01-his-CBM-hutH-B0010

Aly01 here represents a signal peptide used to secrect the fusion protein outside the cell, his-tag enable us to purify the linking protein. The fusion protein CBM-hutH works on the surface of single cell Phaeocystis. globosa and the enzyme catalyzes the reaction of converting histidine to form toxic urocanic acid. His-tag is added to purify the protein. We use BBa_K3739106 to construct the expression system and to express and to purify the protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 653
Illegal NgoMIV site found at 1089
Illegal NgoMIV site found at 1824
Illegal NgoMIV site found at 2060
Illegal AgeI site found at 111
Illegal AgeI site found at 399
Illegal AgeI site found at 489
Illegal AgeI site found at 726
Illegal AgeI site found at 1553
Illegal AgeI site found at 2049
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3739106 short</partinfo>
-Aly01 is a signal peptide from the alginate lyase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid
+Aly01 here represents a signal peptide used to secrect the fusion protein outside the cell, his-tag enable us to purify the linking protein. The fusion protein CBM-hutH works on the surface of single cell Phaeocystis. globosa and the enzyme catalyzes the reaction of converting histidine to form toxic urocanic acid. His-tag is added to purify the protein. We use BBa_K3739106 to construct the expression system and to express and to purify the protein.
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