Difference between revisions of "Part:BBa K3889002"

 
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<partinfo>BBa_K3889002 short</partinfo>
 
<partinfo>BBa_K3889002 short</partinfo>
  
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sfGFP is a variant of Green Fluorescence Protein. It can be used for reporting the expression of the desired promoter.
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sfGFP is a variant of Green Fluorescence Proteinfrom <i>Aequorea victoria</i> and has the following parameters [1]:
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{| class=wikitable
 +
|-
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!Ex : Peak excitation wavelength (in nm)
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|485
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|-
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!Em: Peak emission wavelength (in nm)
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|510
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|-
 +
!EC: Extinction Coefficient  (M<sup>-1</sup> cm<sup>-1</sup>)
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|83,300
 +
|-
 +
!QY: Quantum Yield
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|0.65
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|-
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!Brightness
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|54.15
 +
|-
 +
!Maturation (min)
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|13.6
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|}
 +
 
 +
 
 +
It can be used for reporting the expression of the desired promoter.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889002 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3889002 parameters</partinfo>
 
<partinfo>BBa_K3889002 parameters</partinfo>
 
<!-- -->
 
<!-- -->
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 +
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===References===
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1. https://www.fpbase.org/protein/superfolder-gfp/

Revision as of 14:34, 9 October 2021


modified_sfGFP

sfGFP is a variant of Green Fluorescence Proteinfrom Aequorea victoria and has the following parameters [1]:

Ex : Peak excitation wavelength (in nm) 485
Em: Peak emission wavelength (in nm) 510
EC: Extinction Coefficient (M-1 cm-1) 83,300
QY: Quantum Yield 0.65
Brightness 54.15
Maturation (min) 13.6


It can be used for reporting the expression of the desired promoter.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13



References

1. https://www.fpbase.org/protein/superfolder-gfp/