Difference between revisions of "Part:BBa K3726086:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | The part has been assembled in accordance with the design guidelines of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection. Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence. | |
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===Source=== | ===Source=== | ||
− | + | To know more about Marburg collection guidelines visit https://2018.igem.org/Team:Marburg/Part_Collection | |
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===References=== | ===References=== | ||
+ | [4]V. Mutalik, J. Guimaraes, G. Cambray, C. Lam, M. Christoffersen, Q. Mai, A. Tran, M. Paull, J. Keasling, A. Arkin and D. Endy, "Precise and reliable gene expression via standard transcription and translation initiation elements", Nature Methods, vol. 10, no. 4, pp. 354-360, 2013. |
Latest revision as of 18:01, 8 October 2021
RBS BCD2(Mod)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part has been assembled in accordance with the design guidelines of Marburg Collection https://2018.igem.org/Team:Marburg/Part_Collection. Despite that the part sequence does not include the resulting MoClo Overhangs after level 1 assembly. Then the level 0 part includes the overhangs TACTAGAG in the 5’ end of the sequence, and the bases TAATCAATG in the 3’ end of the sequence.
Source
To know more about Marburg collection guidelines visit https://2018.igem.org/Team:Marburg/Part_Collection
References
[4]V. Mutalik, J. Guimaraes, G. Cambray, C. Lam, M. Christoffersen, Q. Mai, A. Tran, M. Paull, J. Keasling, A. Arkin and D. Endy, "Precise and reliable gene expression via standard transcription and translation initiation elements", Nature Methods, vol. 10, no. 4, pp. 354-360, 2013.